Coding

Part:BBa_K4644006:Design

Designed by: Han Cao   Group: iGEM23_BUCT   (2023-09-24)


sigma32


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 601
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 601
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 601
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 601
    Illegal AgeI site found at 588
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We constructed the pcs-lac-sigma32 plasmid

Source

E.coli

References

Judith Matavacas, Joel Hallgren, Claes von Wachenfeldt, Bacillus subtilis forms twisted cells with cell wall integrity defects upon removal of the molecular chaperones DnaK and trigger factor, Frontiers in Microbiology, 13, (2023).

Gamer, J., Multhaup, G., Tomoyasu, T., McCarty, J.S., Rüdiger, S., Schönfeld, H.J., Schirra, C., Bujard, H. and Bukau, B. (1996), A cycle of binding and release of the DnaK, DnaJ and GrpE chaperones regulates activity of the Escherichia coli heat shock transcription factor sigma32.. The EMBO Journal, 15: 607-617.